Anti-CKIP-1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q53GL0
Gene Id	51177
Gene Symbols	PLEKHO1
Protein Name / Synonyms	CKIP-1 (N-term)
Clonality	Polyclonal
Preparation	This CKIP-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-77 amino acids from the N-terminal region of human CKIP-1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CKIP-1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). This protein may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). It appears to target ATM to the plasma membrane and appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.

Specificity

Predicted Reactivity

Bovine

Other Names

PLEKHO1; CKIP1; Pleckstrin homology domain-containing family O member 1; C-Jun-binding protein; Casein kinase 2-interacting protein 1; Osteoclast maturation-associated gene 120 protein

NCBI Accession #

NP_057358.2

Other Accession #

A4IFK0

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46237

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of CKIP-1 Antibody (N-term) (Cat# 102-17890) in 293 cell line lysates (35ug/lane). CKIP-1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CKIP-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of 293 cells using CKIP-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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