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Anti-CKM Antibody
Catalog #: 130-10054
Rabbit Anti-Human CKM Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P06732 |
| Gene Id | 1158 |
| Gene Symbols | CKM CKMM |
| Clonality | Polyclonal |
| Immunogen | Immunogen was fully length of recombinant expressed human CKM. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 10, 000(at least detecting 100 ng/ml)) |
| Antigen Catalog Number | 230-00038 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Creatine Kinase, Muscle (CKM) is a cytoplasmic enzyme reversibly catalyzing the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). CKM protein belongs to ATP: guanido phosphotransferase protein family. It only presents in skeletal and cardiac muscle tissues at high levels. CKM involves in energy transduction and is an important serum marker for myocardial infarction.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat CKM has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if not be used over months. Minimize freezing and thawing when use.
Related Products
Recombinant Human Creatine Kinase (Muscle) (cat# 230-00038-2) Mouse Anti-Human Creatine Kinase MB (cardiac-specific) (cat# DS-MB-01069 )
References
- Perryman M.B., et al. (1986) Isolation and sequence analysis of a full-length cDNA for human M creatine kinase.Biochem. Biophys. Res. Commun. 140:981-989.
- Trask R.V., et al. (1988) Developmental regulation and tissue-specific expression of the human muscle creatine kinase gene. J. Biol. Chem. 263:17142-17149.
Expiration:
12 months from the date of shipment when stored properly.