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Anti-CKM Antibody, Biotinylated
Catalog #: 130-10054B-50
Rabbit anti-CKM Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06732 |
| Gene Id | 1158 |
| Gene Symbols | CKM CKMM |
| Clonality | Polyclonal |
| Immunogen | Immunogen was fully length of recombinant expressed human CKM. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 10, 000(at least detecting 100 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Creatine Kinase, Muscle (CKM) is a cytoplasmic enzyme reversibly catalyzing the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). CKM protein belongs to ATP: guanido phosphotransferase protein family. It only presents in skeletal and cardiac muscle tissues at high levels. CKM involves in energy transduction and is an important serum marker for myocardial infarction.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat CKM has not yet been tested.
Related Products
Recombinant Human Creatine Kinase (Muscle) (cat# 230-00038-2) Mouse Anti-Human Creatine Kinase MB (cardiac-specific) (cat# DS-MB-01069 )
References
- Perryman M.B., et al. (1986) Isolation and sequence analysis of a full-length cDNA for human M creatine kinase.Biochem. Biophys. Res. Commun. 140:981-989.
- Trask R.V., et al. (1988) Developmental regulation and tissue-specific expression of the human muscle creatine kinase gene. J. Biol. Chem. 263:17142-17149.
Expiration:
12 months from the date of shipment when stored properly.