Synonyms
CACC antibody, CACC1 antibody, CLCRG1 antibody, CaCC-1 antibody, FLJ95147 antibody, GOB5 antibody, hCLCA1 antibody, hCaCC-1 antibody, CLCA1 antibody, calcium-activated chloride channel regulator 1 antibody, "chloride channel, calcium activated, family member 1 antibody", calcium-dependent chloride channel-1 antibody, chloride channel regulator 1 antibody, calcium-activated chloride channel protein 1 antibody, calcium-activated chloride channel family member 1 antibody, "CLCA family member 1, chloride channel regulator antibody", chloride channel accessory 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
100 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 542 and 633 of CLCA1 (Uniprot ID# A8K7I4)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using CLCA1(107-10192) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded gastric CA, using CLCA1(107-10192) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10192 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.