Anti-Cleaved-CASP3 (Asp175) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P42574
Gene Id	836
Gene Symbols	CASP3
Protein Name / Synonyms	Cleaved-CASP3 (Asp175)
Clonality	Polyclonal
Preparation	This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IHC-P, Dot Blotting
Research Area	Apoptosis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CASP3 encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12

NCBI Accession #

NP_004337.2;NP_116786.1

Other Accession #

P55213; Q95ND5; P70677; Q08DY9

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31608

Recommended Dilutions

DB: 1:500; IHC: 1:50-100

	Dot blot analysis of anti-Cleaved-CASP3 (Asp175) Antibody (Cat# 102-15533) on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Cleaved-CASP3 (Asp175)Antibody (Cat# 102-15533) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-CASP3 (Asp175)Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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