Anti-CLIC3 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	9022
Protein Name / Synonyms	CLIC3
Concentration (lot specific)	0.74 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CLIC3 antibody, chloride intracellular channel protein 3 antibody, chloride intracellular channel 3 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

27 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 236 of CLIC3 (Uniprot ID# O95833)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded T(IV)(lung CA), using CLIC3(107-10450) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CLIC3(107-10450) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:HeLa S3
12% SDS PAGE
107-10450 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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