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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95628 |
| Gene Id | 4850 |
| Gene Symbols | CNOT4 |
| Protein Name / Synonyms | CNOT4 |
| Clonality | Polyclonal |
| Preparation | This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein. |
| Concentration (lot specific) | 0.330 mg/ml |
| Recommended Applications | FC, Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
Specificity
Other Names
CNOT4; NOT4; CCR4-NOT transcription complex subunit 4; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp
NCBI Accession #
NP_001008226.1;NP_001177776.1;NP_001177777.1;NP_001177778.1;NP_001177779.1;NP_037448.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
63510
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of CNOT4 Antibody (Cat# 102-12573) in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | CNOT4 Antibody (Cat# 102-12573) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
