Anti-CNOT8 (C-term) Antibody

Product Description

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UFF9
Gene Id	9337
Gene Symbols	CNOT8
Protein Name / Synonyms	CNOT8 (C-term)
Clonality	Polyclonal
Preparation	This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.
Concentration (lot specific)	0.406 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.

Predicted Reactivity

Mouse

Other Names

CNOT8; CALIF; POP2; CCR4-NOT transcription complex subunit 8; CAF1-like protein; CAF2; CCR4-associated factor 8

NCBI Accession #

NP_004770.4

Other Accession #

Q9D8X5

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33540

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50; FC: 1:10-50

	CNOT8 Antibody (C-term) (Cat# 102-16801) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).
	Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term)(Cat# 102-16801).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
	Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HUMAN

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Product Description