Anti-Cofilin 2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	1073
Protein Name / Synonyms	Cofilin 2
Concentration (lot specific)	0.24 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

NEM7 antibody, CFL2 antibody, "cofilin, muscle isoform antibody", cofilin-2 antibody, cofilin 2 (muscle) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

19 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 166 of Cofilin 2 (muscle) (Uniprot ID# Q9Y281)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle)(107-11085) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-11085 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: H1299
B: Molt-4
12% SDS PAGE
107-11085 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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