Anti-COL1A2 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	1278
Protein Name / Synonyms	COL1A2, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

OI4 antibody, COL1A2 antibody, collagen I, alpha-2 polypeptide antibody, collagen of skin, tendon and bone, alpha-2 chain antibody, type I procollagen antibody, collagen alpha-2(I) chain antibody, alpha 2(I)-collagen antibody, alpha-2 type I collagen antibody, collagen, type I, alpha 2 antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

129 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1057 and 1366 of Collagen I alpha2 (Uniprot ID# P08123)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000. WB:Assay dependent. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min.
Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term (107-10358) diluted at 1:500.
Blue: Hoechst 33342 staining.

COL1A2 antibody [C2C3], C-term detects COL1A2 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg whole cell extract of GFP-human COL1A2-transfected 293T cells (partial)
10 % SDS-PAGE
COL1A2 antibody [C2C3], C-term (107-10358) dilution: 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products