Anti-COMMD7 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	149951
Protein Name / Synonyms	COMMD7
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C20orf92 antibody, FLJ14987 antibody, MGC33315 antibody, dJ1085F17.3 antibody, COMMD7 antibody, COMM domain-containing protein 7 antibody, COMM domain containing 7 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

23 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 52 and 142 of COMMD7

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

COMMD7 antibody detects COMMD7 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COMMD7 protein stained by COMMD7 antibody (107-11002) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using COMMD7(107-11002) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: H1299
12% SDS PAGE
107-11002 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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