3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-COTL1 (C-term) Antibody
Catalog #: 102-18043
Rabbit Anti-Human COTL1 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q14019 |
| Gene Id | 23406 |
| Gene Symbols | COTL1 |
| Protein Name / Synonyms | COTL1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This COTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-142 amino acids from the C-terminal region of human COTL1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
Specificity
Predicted Reactivity
Bovine
Other Names
COTL1; CLP; Coactosin-like protein
NCBI Accession #
NP_066972.1
Other Accession #
Q2HJ57; NP_066972
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
15945
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of COTL1 Antibody (C-term) (Cat# 102-18043) in Hela cell line lysates (35ug/lane). COTL1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | COTL1 Antibody (C-term) (Cat# 102-18043) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of COTL1 (arrow) using rabbit polyclonal COTL1 Antibody (C-term) (Cat# 102-18043). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.