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Anti-COX6A1 Antibody
Catalog #: 144-03798
COX6A1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P12074 |
| Gene Id | 1337 |
| Gene Symbols | COX6A1 |
| Protein Name / Synonyms | COX6A1, CMTRID, COX6A, COX6AL, cytochrome c oxidase subunit 6A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented.
Dilutions
WB 1:200 - 1:2000
IHC 1:20 - 1:200
IHC 1:20 - 1:200
Format
Calculated MW
12kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using COX6A1 antibody (144-03798) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using COX6A1 antibody (144-03798) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (144-03798) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (144-03798) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (144-03798) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.