3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-CPSF4 (Center) Antibody
Catalog #: 102-18329
Rabbit Anti-Human CPSF4 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95639 |
| Gene Id | 10898 |
| Gene Symbols | CPSF4 |
| Protein Name / Synonyms | CPSF4 (Center) |
| Clonality | Polyclonal |
| Preparation | This CPSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human CPSF4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.
Specificity
Predicted Reactivity
Rat, Bovine, Xenopus
Other Names
CPSF4; CPSF30; NAR; NEB1; Cleavage and polyadenylation specificity factor subunit 4; Cleavage and polyadenylation specificity factor 30 kDa subunit; NS1 effector domain-binding protein 1; No arches homolog
NCBI Accession #
NP_001075028.1;NP_006684.1
Other Accession #
Q6DJP7; Q5FVR7; O19137
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30255
Recommended Dilutions
WB: 1:1000
 | Western blot analysis of CPSF4 Antibody (Center) (Cat# 102-18329) in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.