Anti-Creatine kinase 1B Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	1159
Protein Name / Synonyms	Creatine kinase 1B
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Phosphorylation
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CKMT antibody, CKMT1 antibody, CKMT1A antibody, UMTCK antibody, CKMT1B antibody, "creatine kinase U-type, mitochondrial antibody", "creatine kinase, mitochondrial 1 (ubiquitous) antibody", ubiquitous mitochondrial creatine kinase antibody, U-MtCK antibody, acidic-type mitochondrial creatine kinase antibody, mia-CK antibody, "creatine kinase, mitochondrial 1B antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

47 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 63 and 287 (P12532) of Creatine kinase 1B

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Creatine kinase 1B(107-10395) antibody at 1:300 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
10% SDS PAGE
107-10395 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products