Creatine kinase (brain) antibody [N3C3], 0.54 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1152 |
| Protein Name / Synonyms | Creatine kinase (brain) |
| Concentration (lot specific) | 0.54 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Phosphorylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
B-CK antibody, CKBB antibody, CKB antibody, creatine kinase B chain antibody, creatine kinase B-type antibody, creatine kinase-B antibody, "creatine kinase, brain antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 165 and 381 of Creatine kinase (brain) (Uniprot ID# P12277)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain)(107-10285) antibody at 1:100 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10285 diluted at 1:10000
A: Mouse brain
10% SDS PAGE
107-10285 diluted at 1:10000
Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10 % SDS-PAGE, and blotted with Creatine kinase (brain) antibody (107-10285) diluted by 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB(107-10285) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:H1299
B:HeLa S3
12% SDS PAGE
107-10285 diluted at 1:1000
A:H1299
B:HeLa S3
12% SDS PAGE
107-10285 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
