Anti-CROT (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9UKG9
Gene Id	54677
Gene Symbols	CROT
Protein Name / Synonyms	CROT (N-term)
Clonality	Polyclonal
Preparation	This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	FC, IHC-P, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Specificity

Other Names

CROT; COT; Peroxisomal carnitine O-octanoyltransferase

NCBI Accession #

NP_001137407.1;NP_001230674.1;NP_066974.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

70178

Recommended Dilutions

FC: 1:10-50; IHC: 1:50-100; WB: 1:1000

	CROT Antibody (N-term)(Cat# 102-18252) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Western blot analysis of CROT Antibody (N-term) (Cat# 102-18252) in K562 cell line and mouse liver tissue lysates (35ug/lane). CROT (arrow) was detected using the purified Pab.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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