Anti-CSB (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	2074
Protein Name / Synonyms	CSB, Internal
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ARMD5 antibody, CKN2 antibody, COFS antibody, COFS1 antibody, CSB antibody, RAD26 antibody, ERCC6 antibody, Cockayne syndrome B protein antibody, ATP-dependent helicase ERCC6 antibody, cockayne syndrome protein CSB antibody, Rad26 homolog antibody, Cockayne syndrome group B protein antibody, DNA excision repair protein ERCC-6 antibody, "excision repair cross-complementing rodent repair deficiency, complementation group 6 antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

168 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 346 and 766 of CSB (Uniprot ID# Q03468)

Applications

Applications Tested

ICC/IF

Application Notes

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (107-10045) dilution: 1:500.

CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (107-10045) dilution: 1:500.

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (107-10045) diluted at 1:500.
Blue: Hoechst 33342 staining.

CSB antibody [N2C1], Internal detects CSB protein by western blot analysis.
A. 30 µg 294T whole cell lysate/extract
B. 30 µg A432 whole cell lysate/extract
C. 31 µg HeLa whole cell lysate/extract
D. 30 µg HepG3 whole cell lysate/extract
5 % SDS-PAGE
CSB antibody [N2C1], Internal (107-10045) dilution: 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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