Synonyms
D1S155E antibody, DKFZp779B0247 antibody, DKFZp779J1455 antibody, FLJ26882 antibody, RP5-1000E10.3 antibody, UNR antibody, CSDE1 antibody, upstream of NRAS antibody, N-ras upstream gene protein antibody, cold shock domain-containing protein E1 antibody, NRAS-related antibody, cold shock domain containing E1, RNA-binding antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
89 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 198 and 433 of CSDE1 (Uniprot ID# O75534)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using CSDE1(107-11171) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using CSDE1(107-11171) antibody at 1:250 dilution.

CSDE1 antibody [N2C1], Internal detects CSDE1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
CSDE1 antibody [N2C1], Internal (107-11171) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.