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Anti-CT1 Antibody, Biotinylated
Catalog #: 130-10013B-50
Rabbit anti-CT1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates Cross reactivity with mouse and rat CT-1 was not tested yet |
| Host Species | Rabbit |
| Accession Number | Q16619 |
| Gene Id | 1489 |
| Gene Symbols | CTF1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was expressing Human CT-1 protein. This antibody was produced from a rabbit immunizedwith the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinitychromatography. |
| Immunogen Sequence | CGLYREWLSRTEGDLGQLLPGGSA |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:800, 000(at least detecting 12.5 µg/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25 µg/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Cardiotrophin-1 (CT-1) is a cardiac hypertrophic factor and belongs to the IL-6 cytokine family.The protein interacts with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR)heterodimer. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstemnuclei of mice. In addition, CT-1 enhances transcription factor NF kappa B DNA-binding activities andactivates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes. Studies have revealed CT-1 ishighly expressed in the heart, prostate, skeletal muscle, and ovary, and expressed at the lower levels inlung, kidney, pancreas, thymus, small intestine, and testis. CT-1 is highly secreted by myotubes, andpromotes the survival of cultured embryonic mouse and rat MNs. CT-1 is associated withpathophysiology of heart diseases, like hypertension, myocardial infarction, valvular heart disease, andcongestive heart failure.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
Related Products
Human Cardiotrophin-1 (CT-1) ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine(Cat# ELH-CT1-001 ); Recombinant Human Cardiotrophin-1 (Cat# 230-00008)
References
- Arce V, et. al. (1998) Synergistic effects of Schwann- and muscle-derived factors on motoneuronsurvival involve GDNF and cardiotrophin-1 (CT-1). J Neurosci 18:1440-1448.
- Ronald W, et al. (2001). Cardiotrophin-1, a muscle-derived Cytokine, is required for the survival ofsubpopulations of developing motoneurons. J Neurosci. 21:1283-1291.
- Arce V, et al. (1999) Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneuronspurified by a novel technique. J Neurosci Res 55:119-126.
Expiration:
12 months from the date of shipment when stored properly.