Anti-CTBP1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13363
Gene Id	1487
Gene Symbols	CTBP1
Protein Name / Synonyms	CTBP1 (C-term)
Clonality	Polyclonal
Preparation	This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Specificity

Other Names

CTBP1; CTBP; C-terminal-binding protein 1

NCBI Accession #

NP_001012632.1;NP_001319.1

Other Accession #

NP_001319.1; NP_001012632.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

47535

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	CTBP1 Antibody (C-term) (Cat# 102-12807) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).
	Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term)(Cat# 102-12807). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.
	Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term)(Cat# 102-12807). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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