Anti-CTDSP1-V250 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9GZU7
Gene Id	58190
Gene Symbols	CTDSP1
Protein Name / Synonyms	CTDSP1-V250 (C-term)
Clonality	Polyclonal
Preparation	This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.

Specificity

Predicted Reactivity

Mouse

Other Names

CTDSP1; NIF3; NLIIF; SCP1; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; Nuclear LIM interactor-interacting factor 3; Small C-terminal domain phosphatase 1

NCBI Accession #

NP_001193807.1;NP_067021.1;NP_872580.1

Other Accession #

P58466

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

29203

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-CTDSP1-V250 (Cat# 102-17885) is used in Western blot to detect CTDSP1-V250 in CEM tissue lysate.
	Western blot analysis of CTDSP1 (arrow) using CTDSP1-V250 Antibody (C-term) (Cat# 102-17885). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTDSP1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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