Anti-CUL5 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q93034
Gene Id	8065
Gene Symbols	CUL5
Protein Name / Synonyms	CUL5 (C-term)
Clonality	Polyclonal
Preparation	This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 747-774 amino acids from the C-terminal region of human CUL5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, FC, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.

Specificity

Predicted Reactivity

Rabbit

Other Names

CUL5; VACM1; Cullin-5; Vasopressin-activated calcium-mobilizing receptor 1

NCBI Accession #

NP_003469.2

Other Accession #

Q29425

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

90955

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of anti-CUL5 Antibody (C-term)(Cat# 102-17427) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab.
	Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	CUL5 Antibody (C-term) (Cat# 102-17427) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUL5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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