Anti-Cullin 2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13617
Gene Id	8453
Gene Symbols	CUL2
Protein Name / Synonyms	Cullin-2, CUL-2, CUL2
Clonality	Polyclonal
Recommended Applications	Western Blotting, IF, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Other Information

Antigen Type

Synthetic Peptide

Images

WB ( 1:1000)

Western blot analysis of lysate from MDA-MB-231 cell line,using Cullin 2 Antibody(C0163). C0163 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.

IF (1:100)

Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody .

IHC (1:50-1:100)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody .

WB (1:1000)

Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 Antibody. The lane on the left is treated with systhesized peptide.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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