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Anti-Cyclin D1 (CCND1 ) Antibody
Catalog #: 130-10126
Rabbit Anti- Cyclin D1(CCND1 ) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAX36345 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein CCND1.The membrane blot was probed with antiCCND1 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein CCND1 from E.coli cell (35kDa). |
| Antigen Catalog Number | 230-00261 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cell Cycle |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Cyclin D1 (CCND1) is a critical mitogen-regulated cell-cycle control element whose transcriptional modulation plays a crucial role in cancer growth and progression. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat CCND1 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Mouse Anti-Human Cyclin D1 (Cat: DS-MB-01076)
References
- Elena Beltran, et al. (2011) A cyclin-D1 interaction with BAX underlies its oncogenic role and potential as a therapeutic target in mantle cell lymphoma. Medical Sciences.26; 108(30): 1246112466.
- Tomoharu Kanie, et al. (2012) Genetic Reevaluation of the Role of F-Box Proteins in Cyclin D1 Degradation. Mol Cell Biol. 32(3): 590605.
Expiration:
12 months from the date of shipment when stored properly.