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Anti-CYCS Antibody
Catalog #: 144-60334
CYCS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P99999 |
| Gene Id | 54205 |
| Gene Symbols | CYCS |
| Protein Name / Synonyms | CYCS, CYC, HCS, THC4, cytochrome c |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
14kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human CYCS (NP_061820.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CYCS antibody (144-60334) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using CYCS antibody (144-60334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using CYCS antibody (144-60334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CYCS antibody (144-60334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CYCS antibody (144-60334) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using CYCS antibody (144-60334) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.