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Anti-CYFRA21-1 Antibody, Biotinylated
Catalog #: RB-16-0001B-50
Rabbit anti-CYFRA21-1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_000406 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from functional epitope of CYFRA21-1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulfate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 0.1-0.4 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
CYFRA21-1 is an antigenic determinant which represents a fragment of the 40kD protein Cytokeratin 19. This antigen is defined and measured in serum by using two mouse monoclonal antibodies; KS 19-1 and BM 19-21. Both are reactive to epitopes 1 and 21 of cytokeratin 19 (thus this cytokeratin 19 fragment is named as CYFRA21-1). CYFRA21-1 is a tumor marker based on the determination of water-soluble cytokeratin 19 which is secreted by normal or malignantly transformed epithelial cells.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti- CYFRA21-1 antibody detects both synthetic peptide and cellular proteins from tested cancer cell lines (Hela, HCF-7 and Hep G2). No cross reactivity with other proteins was found.
References
- Stieber P, Zimmermann A, Reinmiedl J, M¼ller C, Hoffmann H, Dienemann H. CYFRA 21-1 in the early diagnosis of recurrent disease in non small cell lung carcinomas (NSCLC). Anticancer Res (1999)
- Pujol JL, Molinier O, Ebert W, Daur¨s JP, Barlesi F, Buccheri G, Paesmans M, Quoix E, Moro-Sibilot D, Szturmowicz M, Br©chot JM, Muley T, Grenier J. CYFRA 21-1 is a prognostic determinant in nonsmall-cell lung cancer: results of a meta-analysis in 2063 patients. Br J Cancer (2004)
Expiration:
12 months from the date of shipment when stored properly.