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Anti-CYFRA21-1 Antibody
Catalog #: RB-16-0001
Rabbit Anti-Human CYFRA21-1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_000406 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from functional epitope of CYFRA21-1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulfate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 0.1-0.4 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, like Sodium Azide. The final concentration is indicated on shipping vial. The antibody is stable for at least a year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggested with sterile water) can also be aliquoted and stored frozen at -20°C to - 70°C in a manual defrost freezer for months without detectable loss of activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
CYFRA21-1 is an antigenic determinant which represents a fragment of the 40kD protein Cytokeratin 19. This antigen is defined and measured in serum by using two mouse monoclonal antibodies; KS 19-1 and BM 19-21. Both are reactive to epitopes 1 and 21 of cytokeratin 19 (thus this cytokeratin 19 fragment is named as CYFRA21-1). CYFRA21-1 is a tumor marker based on the determination of water-soluble cytokeratin 19 which is secreted by normal or malignantly transformed epithelial cells.
Specificity
The rabbit anti- CYFRA21-1 antibody detects both synthetic peptide and cellular proteins from tested cancer cell lines (Hela, HCF-7 and Hep G2). No cross reactivity with other proteins was found.
Storage
Store at -20°C if not intended for use within a month. May keep at 4°C for a month but minimize freezing and thawing during use.
References
- Stieber P, Zimmermann A, Reinmiedl J, Müller C, Hoffmann H, Dienemann H. CYFRA 21-1 in the early diagnosis of recurrent disease in non small cell lung carcinomas (NSCLC). Anticancer Res (1999)
- Pujol JL, Molinier O, Ebert W, Daurès JP, Barlesi F, Buccheri G, Paesmans M, Quoix E, Moro-Sibilot D, Szturmowicz M, Bréchot JM, Muley T, Grenier J. CYFRA 21-1 is a prognostic determinant in nonsmall-cell lung cancer: results of a meta-analysis in 2063 patients. Br J Cancer (2004)
Expiration:
12 months from the date of shipment when stored properly.