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Anti-CYFRA21-1 (N-term) Antibody, Biotinylated

Catalog #: RB-16-0004B-50

Rabbit anti-CYFRA21-1 (N-terminus) Biotinylated Antibody, 50 ug

Product Description

Specifications

Size50 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
Accession Number
NP_000406
ClonalityPolyclonal
Immunogen
Immunogen was synthetic peptide derived from functional epitope of CYFRA21-1. This antibody was produced from a rabbit. The IgG fraction was purified from rabbit serum by ammonium sulfate precipitation followed by Protein A/G affinity chromatography.
IsotypeIgG
Recommended ApplicationsELISA (0.1 - 0.4 &micro µg/ml)
Reconstitution
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

CYFRA21-2 is an antigenic determinant which represents a fragment of the 40kD protein Cytokeratin 19. This antigen is defined and measured in serum by using two mouse monoclonal antibodies; KS 19-1 and BM 19-21. Both are reactive to epitopes 1 and 21 of cytokeratin 19 (thus this cytokeratin 19 fragment is named as CYFRA21-1). CYFRA21-1 is a tumor marker based on the determination of water-soluble cytokeratin 19 which is secreted by normal or malignantly transformed epithelial cells.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Specificity

This antibody detects both synthetic peptide and cellular proteins from tested cancer cell lines (Hela, HCF-7 and Hep G2). No cross reactivity with other proteins was found.

Storage/Stability

Store at -20°C if not intended for use within a month. May keep at 4°C for a month. Minimize freezing and thawing during use.

The antibody is stable for at least a year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggested with steril PBS) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss of activity. Upon reconstitution, the antibody can be stored over a month at 4°C.
Expiration:
12 months from the date of shipment when stored properly.