Anti-CYP21A2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08686
Gene Id	1589
Gene Symbols	CYP21A2
Protein Name / Synonyms	CYP21A2 (Center)
Clonality	Polyclonal
Preparation	This CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the Central region of human CYP21A2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.

Specificity

Other Names

CYP21A2; CYP21; CYP21B; Steroid 21-hydroxylase; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21; Cytochrome P450-C21B

NCBI Accession #

NP_000491.4;NP_001122062.3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55887

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of CYP21A2 Antibody (Center) (Cat# 102-17679) in MDA-MB435 cell line lysates (35ug/lane). CYP21A2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CYP21A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	CYP21A2 Antibody (Center) (Cat# 102-17679) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of CYP21A2 Antibody (Center)(Cat# 102-17679) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products