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Anti-CYP2E1 Antibody
Catalog #: 144-61331
CYP2E1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P05181 |
| Gene Id | 1571 |
| Gene Symbols | CYP2E1 |
| Protein Name / Synonyms | CYP2E1, CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:100
IHC 1:50 - 1:100
IF 1:20 - 1:100
Format
Calculated MW
56kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CYP2E1 Antibody (144-61331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody (144-61331) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody (144-61331) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CYP2E1 antibody (144-61331). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.