Anti-CYP3A5 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P20815
Gene Id	1577
Gene Symbols	CYP3A5
Protein Name / Synonyms	CYP3A5 (C-term)
Clonality	Polyclonal
Preparation	This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.

Specificity

Other Names

CYP3A5; Cytochrome P450 3A5; CYPIIIA5; Cytochrome P450 HLp2; Cytochrome P450-PCN3

NCBI Accession #

NP_000768.1;NP_001177413.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

57109

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term) (Cat# 102-17627). 102-17627 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Western blot analysis of anti-CYP3A5 Antibody (C-term) (Cat# 102-17627) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term)(Cat# 102-17627) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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