Synonyms
CP4Y antibody, CYP4A2 antibody, CYP4AII antibody, CYP4A11 antibody, 20-hydroxyeicosatetraenoic acid synthase antibody, cytochrome P450HL-omega antibody, "cytochrome P450, subfamily IVA, polypeptide 11 antibody", CYPIVA11 antibody, fatty acid omega-hydroxylase antibody, cytochrome P-450HK-omega antibody, 20-HETE synthase antibody, cytochrome P450 4A11 antibody, alkane-1 monooxygenase antibody, P450HL-omega antibody, lauric acid omega-hydroxylase antibody, "cytochrome P450, family 4, subfamily A, polypeptide 11 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 48 and 316 of CYP4A11 (Uniprot ID# Q02928)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytochrome P450 4A11(107-10388) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded mixed OVCA, using CYP4A11(107-10388) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:A431
B:H1299
7.5% SDS PAGE
107-10388 diluted at 1:100
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.