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Anti-CYP51A1 (Center) Antibody
Catalog #: 102-18061
Rabbit Anti-Human CYP51A1 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16850 |
| Gene Id | 1595 |
| Gene Symbols | CYP51A1 |
| Protein Name / Synonyms | CYP51A1 (Center) |
| Clonality | Polyclonal |
| Preparation | This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human CYP51A1. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | IHC-P, FC, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.
Specificity
Predicted Reactivity
Monkey
Other Names
CYP51A1; CYP51; Lanosterol 14-alpha demethylase; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P450LI; Sterol 14-alpha demethylase
NCBI Accession #
NP_000777.1;NP_001139624.1
Other Accession #
Q4R8S6
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
56806
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of lysate from LNCap cell line, using CYP51A1 Antibody (Center)(Cat# 102-18061). 102-18061 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
 | Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody (Center) (Cat# 102-18061), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | CYP51A1 Antibody (Center) (Cat# 102-18061) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.