Anti-CYP8B1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UNU6
Gene Id	1582
Gene Symbols	CYP8B1
Protein Name / Synonyms	CYP8B1 (C-term)
Clonality	Polyclonal
Preparation	This CYP8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human CYP8B1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	FC, Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.

Specificity

Other Names

CYP8B1; CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase

NCBI Accession #

NP_004382.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58068

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of CYP8B1 Antibody (C-term) (Cat# 102-18029) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	CYP8B1 Antibody (C-term) (Cat# 102-18029) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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