Anti-Cytokeratin 13 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	3860
Protein Name / Synonyms	Cytokeratin 13
Concentration (lot specific)	0.59 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CK13 antibody, K13 antibody, MGC161462 antibody, MGC3781 antibody, KRT13 antibody, cytokeratin 13 antibody, keratin-13 antibody, keratin, type I cytoskeletal 13 antibody, CK-13 antibody, cytokeratin-13 antibody, keratin 13 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

50 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 198 and 438 of Cytokeratin 13

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13(107-10876) antibody at 1:500 dilution.

Cytokeratin 13 antibody detects KRT13 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
10 % SDS-PAGE
Cytokeratin 13 antibody (107-10876) dilution: 1:10000

Cytokeratin 13 antibody detects Cytokeratin 13 protein at cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 13 antibody (107-10876) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13(107-10876) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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