Anti-Cytokeratin 20 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	54474
Protein Name / Synonyms	Cytokeratin 20, Internal
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, MGC35423 antibody, KRT20 antibody, cytokeratin-20 antibody, keratin-20 antibody, cytokeratin 20 antibody, protein IT antibody, "keratin, type I cytoskeletal 20 antibody", keratin 20 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

48 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 75 and 317 of Cytokeratin 20 (Uniprot ID# P35900)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(107-10929) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(107-10929) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-10929 diluted at 1:3000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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