Anti-DAP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P51397
Gene Id	1611
Gene Symbols	DAP
Protein Name / Synonyms	DAP, death associated protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100

Format

Calculated MW

11kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DAP antibody (144-06150) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - DAP Polyclonal Antibody (144-06150)

Immunohistochemistry of paraffin-embedded rat kidney using DAP antibody (144-06150) at dilution of 1:200 (40x lens).
Immunohistochemistry - DAP Polyclonal Antibody (144-06150)

Immunofluorescence analysis of MCF-7 cells using DAP antibody (144-06150). Blue: DAPI for nuclear staining.
Immunofluorescence - DAP Polyclonal Antibody (144-06150)

Expiration:

12 months from the date of shipment when stored properly.

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