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Anti-DCK Antibody

Catalog #: 107-10695

DCK antibody, 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Gene Id
1633
Protein Name / Synonyms
DCK
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

MGC117410 antibody, MGC138632 antibody, DCK antibody, deoxycytidine kinase antibody, deoxynucleoside kinase antibody

Specificity

Species Reactivity
Human, Rat, Mouse

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 260 of DCK (Uniprot ID# P27707)

Applications

Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: DCK antibody (107-10695) dilution: 1:250.
Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10695 diluted at 1:5000
DCK antibody detects DCK protein by western blot analysis.
A. 50 µg mouse brain extract
12 % SDS-PAGE
DCK antibody (107-10695) dilution: 1:1000
DCK antibody detects DCK protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
B. 30 µg Rat2 whole cell extract
12 % SDS-PAGE
DCK antibody (107-10695) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase(107-10695) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.