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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 1639 |
| Protein Name / Synonyms | DCTN1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DAP-150 antibody, DP-150 antibody, HMN7B antibody, P135 antibody, DCTN1 antibody, dynactin subunit 1 antibody, 150 kDa dynein-associated polypeptide antibody, "dynactin 1 (p150, glued homolog, Drosophila) antibody", dynactin 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
142 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1216 and 1278 of DCTN1 (Uniprot ID# Q14203)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1(107-10193) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded OVCA, using dynactin 1(107-10193) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: JurKat
5% SDS PAGE
107-10193 diluted at 1:3000
A: JurKat
5% SDS PAGE
107-10193 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
