Anti-DDAH1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O94760
Gene Id	23576
Gene Symbols	DDAH1
Protein Name / Synonyms	DDAH1 (C-term)
Clonality	Polyclonal
Preparation	This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IHC-P, Western Blotting, FC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

DDAH1; DDAH; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1

NCBI Accession #

NP_001127917.1;NP_036269.1

Other Accession #

O08557; Q9CWS0; P56965

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31122

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Western blot analysis of DDAH1 Antibody (C-term) (Cat# 102-15214) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.
	DDAH1 Antibody (C-term) (Cat# 102-15214) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of DDAH1 Antibody (C-term)(Cat# 102-15214) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products