Anti-DDB1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	1642
Protein Name / Synonyms	DDB1
Concentration (lot specific)	0.81 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, DNA damage-binding protein a antibody, XPE-binding factor antibody, UV-DDB 1 antibody, xeroderma pigmentosum group E-complementing protein antibody, UV-damaged DNA-binding protein 1 antibody, DNA damage-binding protein 1 antibody, XAP-1 antibody, HBV X-associated protein 1 antibody, DDB p127 subunit antibody, UV-damaged DNA-binding factor antibody, damage-specific DNA-binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

127 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 749 and 1140 of DDB1 (Uniprot ID# Q16531)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 µg of DDB1 antibody (107-10185) 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody (107-10185) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10185 diluted at 1:5000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1(107-10185) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1(107-10185) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10185 diluted at 1:1000

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

107-10185 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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