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Anti-DDX3 Antibody
Catalog #: 107-10863
DDX3 antibody, 0.77 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8653 |
| Protein Name / Synonyms | DDX3 |
| Concentration (lot specific) | 0.77 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
DBY antibody, DDX3Y antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome antibody, DEAD box protein 3, Y-chromosomal antibody, ATP-dependent RNA helicase DDX3Y antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
73 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 70 and 346 of DDX3 (Uniprot ID# O15523)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:20000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 30 µg Jurkat
B. 30 µg Raji
C. 30 µg NCI-H929
7.5 % SDS-PAGE
DDX3 antibody (107-10863) dilution: 1:10000
A. 30 µg Jurkat
B. 30 µg Raji
C. 30 µg NCI-H929
7.5 % SDS-PAGE
DDX3 antibody (107-10863) dilution: 1:10000
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10863 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-10863 diluted at 1:1000
DDX3 antibody detects DDX3Y protein by Western blot analysis.
A. 50 µg Rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (107-10863) dilution: 1:1000
A. 50 µg Rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (107-10863) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3Y(107-10863) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2
7.5% SDS PAGE
107-10863 diluted at 1:10000
A: H1299
B: Hep G2
7.5% SDS PAGE
107-10863 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.