3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-DDX39 Antibody
Catalog #: 107-10579
DDX39 antibody [N1C3], 0.96 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10212 |
| Protein Name / Synonyms | DDX39 |
| Concentration (lot specific) | 0.96 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
BAT1 antibody, BAT1L antibody, DDX39 antibody, DDXL antibody, MGC18203 antibody, MGC8417 antibody, URH49 antibody, DDX39A antibody, "nuclear RNA helicase, DECD variant of DEAD box family antibody", DEAD box protein 39 antibody, "UAP56-related helicase, 49 kDa antibody", DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 transcript antibody, ATP-dependent RNA helicase DDX39A antibody, nuclear RNA helicase URH49 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
49 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 53 and 425 of DDX39 (Uniprot ID# O00148)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX39(107-10579) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded gastric CA, using DDX39(107-10579) antibody at 1:100 dilution.
Sample(30µg whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
10% SDS PAGE
107-10579 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
10% SDS PAGE
107-10579 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.