RayBiotech

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ISO 13485:2016 cGMP

Anti-DDX43 Antibody

Catalog #: 144-61247

DDX43 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesMouse, Rat
Host SpeciesRabbit
Accession Number
Q9NXZ2
Gene Id
55510
Gene Symbols
DDX43
Protein Name / Synonyms
DDX43, CT13, HAGE, DEAD-box helicase 43
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
21kDa/72kDa
Observed MW
73kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 429-648 of human DDX43 (NP_061135.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DDX43 antibody (144-015858) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - DDX43 antibody (144-015858)

Expiration:
12 months from the date of shipment when stored properly.