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Anti-DDX56 Antibody
Catalog #: 107-11166
DDX56 antibody, 0.96 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 54606 |
| Protein Name / Synonyms | DDX56 |
| Concentration (lot specific) | 0.96 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
DDX21 antibody, DDX26 antibody, NOH61 antibody, DDX56 antibody, ATP-dependent 61 kDa nucleolar RNA helicase antibody, 61-kd nucleolar helicase antibody, putative nucleolar RNA helicase antibody, DEAD box protein 21 antibody, DEAD-box RNA helicase antibody, probable ATP-dependent RNA helicase DDX56 antibody, DEAD box protein 56 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
62 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 201 and 393 of DDX56
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56(107-11166) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using DDX56(107-11166) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-11166 diluted at 1:1000
A: Hela
7.5% SDS PAGE
107-11166 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.