Synonyms
C1ORF18 antibody, C1orf218 antibody, DKFZp547O0715 antibody, FAM31B antibody, FLJ20054 antibody, FLJ21179 antibody, FLJ33888 antibody, MGC103810 antibody, MGC27044 antibody, DENND1B antibody, "family with sequence similarity 31, member B antibody", DENN domain-containing protein 1B antibody, connecdenn 2 antibody, DENN/MADD domain containing 1B antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
48 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 272 of DENND1B (Uniprot ID# Q6P3S1)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10672 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B(107-10672) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: MOLT4
7.5% SDS PAGE
107-10672 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.