Rabbit anti-Der P1 Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Dermatophagoides pteronyssinus (European house dust mite) |
| Host Species | Rabbit |
| Accession Number | P08176 |
| Gene Symbols | DERP1 |
| Clonality | Polyclonal |
| Immunogen | This antibody was produced from a rabbit immunized with the immunogen, expressed protein. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50 µg/ml)), Western Blotting (recommended work dilution= 1:1, 000-5, 000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. The membrane blot was probed with anti-Der P1 primary antibody (0.25 µg/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate recombinant immunogen Der P1 (15kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
DERP1 is a thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues. DERP1 is a C1 peptidase family member. DERP1 has extensive endopeptidase specificity. DERP1 is N-glycosylated. N-glycanase treatment does not completely remove carbohydrates, suggesting that the protein contains additional glycosylation sites. DERP1 causes an allergic reaction in humans. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis. DER P1 binds to IgE in 80% of patients with house dust allergy
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-Der P1 antibody detects specifically target derived from recombinant protein at various concentrations.
Related Products
Recombinant Der P1 Protein (Cat# 228-10319-3)
References
- O'Brien R, Ooi MA, Clarke AH, Thomas WR. Immunologic responses following respiratory sensitization to house dust mite allergens in mice.(1996) Immunol Cell Biol.74(2):174-9.
- Chen KW, Fuchs G, Sonneck K, Gieras A, Swoboda I, Douladiris N, Linhart B, Jankovic M, Pavkov T, Keller W, Papadopoulos NG, Valent P, Valenta R, Vrtala S. Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering.(2008) Mol Immunol. 45(9):2486-98.
Expiration:
12 months from the date of shipment when stored properly.
