Synonyms
ARVD11 antibody, CDHF2 antibody, DG2 antibody, DGII/III antibody, DKFZp686I11137 antibody, DSC3 antibody, DSC2 antibody, desmosomal glycoprotein II antibody, cadherin family member 2 antibody, desmosomal glycoprotein III antibody, desmocollin-2 antibody, desmosomal glycoprotein II/III antibody, desmocollin 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
100 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 480 and 706 of Desmocollin 2 (Uniprot ID# Q02487)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Desmocollin 2 antibody [C1C2], Internal detects Desmocollin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5 % SDS-PAGE, and blotted with Desmocollin 2 antibody [C1C2], Internal (107-10804) diluted by 1:500

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Desmocollin 2 (107-10804) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.