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Anti-DFFA (C-term) Antibody
Catalog #: 102-18218
Rabbit Anti-Human DFFA (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O00273 |
| Gene Id | 1676 |
| Gene Symbols | DFFA |
| Protein Name / Synonyms | DFFA (C-term) |
| Clonality | Polyclonal |
| Preparation | This DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human DFFA. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Specificity
Other Names
DFFA; DFF1; DFF45; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD
NCBI Accession #
NP_004392.1;NP_998731.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
36522
Recommended Dilutions
WB: 1:1000
 | Western blot analysis of DFFA (arrow) using rabbit polyclonal DFFA Antibody (C-term) (Cat# 102-18218). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DFFA gene. |
 | Western blot analysis of DFFA Antibody (C-term) (Cat# 102-18218) in K562 cell line lysates (35ug/lane). DFFA (arrow) was detected using the purified Pab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.